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Principles of Protein Engineering - Christin Peters - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Principles of Protein Engineering - Christin Peters - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Comprehensive Reference Work Detailing Basic and Advanced Information Surrounding Protein Engineering Protein engineering is a method of changing a protein sequence to achieve a desired result, such as a change in the substrate specificity or increased stability to the temperature, organic solvents, and/or extremes of pH or selectivity in catalytic reactions. Principles of Protein Engineering provides researchers the necessary fundamental knowledge about the protein engineering field and the rationale behind the techniques. Written by two experts with significant first-hand experience in the field, the work provides insight on protein synthesis, expression, structure elucidation, bioinformatics, directed evolution, stability, screening, and rational design. To aid in reader comprehension, the text is enhanced by short comments, feedback and personal anecdotes from pioneers and leaders in the field. Sample ideas covered in the work include: - - The fundamental knowledge of protein synthesis and expression - - Basic knowledge about alignment, homology modelling, dynamics of structure, and modeling enzyme kinetics - - The main protein engineering strategies, with a focus on directed evolution and rational design - - Differences between screening and selection, plus the main techniques for high-throughput screening Biochemists, biotechnologists, molecular biologists, and students in related programs of study can use Principles of Protein Engineering to attain both holistic and specific insight into the field of protein engineering and its numerous applications.

DKK 1040.00
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Protein Chromatography - Giorgio Carta - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Protein Chromatography - Giorgio Carta - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.

DKK 1030.00
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Advanced Chemical Biology - - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Advanced Chemical Biology - - Bog - Wiley-VCH Verlag GmbH - Plusbog.dk

Advanced Chemical Biology The modern approach to teaching chemical biology Advanced Chemical Biology is organized around the central dogma of life, progressing from genes to proteins and higher-order cellular structures, including core application areas such as imaging, chemical genetics, activity-based protein profiling, and natural product discovery and biosynthesis. Advanced topics and applications in, e. g., microbiology, developmental biology, and neurobiology, are covered in separate sections. Every chapter is homogeneous in style and layout, consisting of a short historical introduction followed by a description of the underlying concepts and a selection of recent examples of how the concept has been turned into practice. The subdivision of the contents into core and supplemental chapters enables a flexible use in teaching, both for a one-semester and a two-semester course. Written by authors and editors coming from the leading scientific institutions that have developed the concepts and technologies for this discipline, Advanced Chemical Biology includes specific information on topics like: DNA function, synthesis and engineering, chemical approaches to genome integrity, and RNA function, synthesis, and probingChemical approaches to transcription and RNA regulation in vivo, chemical biology of genome engineering, and peptide/protein synthesis and engineeringDirected evolution for chemical biology, chemical biology of cellular metabolism, chemical biology of lipids, and protein post-translational modificationsChemical glycobiology, chemical and enzymatic modification of proteins, genetic code expansion, bio-orthogonal chemistry, and cellular imaging With its broad scope and focus on turning concepts into applications, Advanced Chemical Biology is an excellent starting point for anyone entering the field and looking for a guide to the wide range of available methods and strategies that chemical biology has to offer. With a Foreword by Nobel Laureate Carolyn Bertozzi.

DKK 982.00
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