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Forensic DNA Transfer

Forensic DNA Transfer

Forensic DNA Transfer provides a guide to the recognition and current understanding of DNA transfer in forensic criminal investigations. Increased improvements in technology mean that it is now routinely possible to obtain DNA profiles from non-visible deposits. How or when the DNA in question was deposited may be an issue in the context of the case especially if the donor of the DNA is not in dispute. A DNA profile alone cannot reveal when or how that DNA was deposited at a crime scene nor can it reveal the body matter from which it originated. Issues of transfer associated with activities may be debated—which the traditional discrimination purpose of DNA profiling cannot address. DNA may be everywhere and anywhere—in homes at workplaces during transport and on personal items including clothing. DNA from a person may be on an object they have never contacted or in a room they have never entered. Concepts discussed in the book include non-self DNA on hands through day-to-day activities the prevalence of background DNA in the environment and perhaps on the exhibit the persistence of any DNA transferred and that a DNA result will depend on these variables as well as recovery techniques. Since DNA may be transferred to an exhibit: (a) during the commission of a crime (b) before the crime and/or (c) after the crime through handling examination and testing this book covers various transfer pathways and sources of DNA. Documented cases of inadvertent transfer of DNA resulting in wrongful convictions or the misdirection of criminal investigations are discussed—with an emphasis on contamination mitigation throughout the entire process. Forensic DNA Transfer examines the additional complexity resulting from non-visible deposits of DNA that impact on sampling and testing regimes. The changing understanding of the composition of purported 'touch DNA' deposits from the skin including extracellular DNA transported via body secretions is described. Further the newer focus on interpreting DNA evidence—using activity level propositions and the rationale and associated issues—is also discussed.

GBP 46.99
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Forensic DNA Applications An Interdisciplinary Perspective

Forensic DNA Applications An Interdisciplinary Perspective

Forensic DNA Applications: An Interdisciplinary Perspective Second Edition is fully updated to outline the latest advances in forensic DNA testing techniques and applications. It continues to fill the need for a reference book for people working in the field of forensic molecular biology testing and research as well as individuals investigating and adjudicating cases involving DNA evidence whether they be civil or criminal cases. DNA techniques have greatly impacted obvious traditional forensic areas but such advances have also positively affected myriad new areas of research and inquiry. It is possible today to think about solving forensic problems that were simply unheard of even a few years ago. As such the book pulls all relevant research and applied science together into a detailed and comprehensive collection. Part I begins with the history and development of DNA typing and profiling for criminal and civil purposes. It discusses the statistical interpretation of results with case examples mitochondrial DNA testing Y single nucleotide polymorphisms (SNPs) and short tandem repeats (STRs) and X SNP and STR testing. It also explores low copy number DNA typing mixtures and quality assurance and control. Part II moves on to cover the various uses and applications of analyzing collected physical evidence victim identification in mass disasters analyzing animal DNA forensic botany and other unique applications. Part III is dedicated to the latest advances and developments in human molecular biology and Part IV looks at policies and laws and ethics governing DNA evidence and its utilization in various cases and the courts. Forensic DNA Applications Second Edition covers cutting-edge research and advancements in the field and is the most up-to-date reference available. Edited and contributed to by the world's foremost leaders in the field it is a must-have reference for established professionals and an essential resource to legal professionals—lawyers and judges dealing with civil and criminal cases involving DNA technology—as well as students entering the fields of genetics and forensic DNA analysis. | Forensic DNA Applications An Interdisciplinary Perspective

GBP 56.99
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Artificial DNA Methods and Applications

Artificial DNA Methods and Applications

Combining elements of biochemistry molecular biology and immunology artificial DNA can be employed in a number of scientific disciplines. Some of the varied applications include site-specific mutagenesis hybridization amplification protein engineering anti-sense technology DNA vaccines protein vaccines recombinant antibodies screening for genetic and pathogenic diseases development of materials with new biochemical and structural properties and many more. Artificial DNA: Methods and Applications introduces the concept of artificial DNA that has been rationally designed and explains how it may be exploited in order to develop products that will achieve your intended purpose. The first part of the book covers methods of oligonucleotide synthesis and direct applications of synthetic DNA. The second part describes methods of gene assembly from synthetic oligonucleotides and applications of synthetic genes. The authors also discuss the different trends and future developments within each application area . With state-of-the art research the contributing authors describe how to engineer proteins using rational and semi-rational design to exhibit the desired traits and detail the various amplification reactions and hybridization techniques for modeling evolution and for use in basic research. The only text devoted to this subject Artificial DNA offers a comprehensive review that allows you to understand the strategy design and applications of synthetic oligonucleotides. | Artificial DNA Methods and Applications

GBP 59.99
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Forensic DNA Analysis Current Practices and Emerging Technologies

Forensic DNA Analysis Current Practices and Emerging Technologies

The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing forensic casework family lineage studies identification of human remains and DNA databasing. Forensic DNA Analysis: Current Practices and Emerging Technologies explores the fundamental principles and the application of technologies for each aspect of forensic DNA analysis. The book begins by discussing the value of DNA evidence and how to properly recognize document collect and store it. The remaining chapters examine:The most widely adopted methods and the best practices for DNA isolation from forensic biological samples and human remainsStudies carried out on the use of both messenger RNA and small (micro) RNA profilingReal-time polymerase chain reaction (PCR) methods for quantification and assessment of human DNA prior to genotypingCapillary electrophoresis (CE) as a tool for forensic DNA analysisNext-generation short tandem repeat (STR) genotyping kits for forensic applications the biological nature of STR loci and Y-chromosome STRs (Y-STRs)Mitochondrial DNA (mtDNA) sequence analysisSingle nucleotide polymorphisms (SNPs) and insertion/deletion polymorphisms (indels) in typing highly degraded DNADeep-sequencing technologiesThe current state of integrated systems in forensic DNA analysis The book concludes by discussing various aspects of sample-processing training and the entities that provide such training programs. This volume is an essential resource for students researchers teaching faculties and other professionals interested in human identification/forensic DNA analysis. | Forensic DNA Analysis Current Practices and Emerging Technologies

GBP 77.99
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Forensic DNA Profiling A Practical Guide to Assigning Likelihood Ratios

Forensic DNA Profiling A Practical Guide to Assigning Likelihood Ratios

DNA testing and its forensic analysis are recognized as the “gold standard” in forensic identification science methods. However there is a great need for a hands-on step-by-step guide to teach the forensic DNA community how to interpret DNA mixtures how to assign a likelihood ratio and how to use the subsequent likelihood ratio when reporting interpretation conclusions. Forensic DNA Profiling: A Practical Guide to Assigning Likelihood Ratios will provide a roadmap for labs all over the world and the next generation of analysts who need this foundational understanding. The techniques used in forensic DNA analysis are based upon the accepted principles of molecular biology. The interpretation of a good-quality DNA profile generated from a crime scene stain from a single-source donor provides an unambiguous result when using the most modern forensic DNA methods. Unfortunately many crime scene profiles are not single source. They are described as mixed since they contain DNA from two or more individuals. Interpretation of DNA mixtures represents one of the greatest challenges to the forensic DNA analyst. As such the book introduces terms used to describe DNA profiles and profile interpretation. Chapters explain DNA extraction methods the polymerase chain reaction (PCR) capillary electrophoresis (CE) likelihood ratios (LRs) and their interpretation and population genetic models—including Mendelian inheritance and Hardy-Weinberg equilibrium. It is important that analysts understand how LRs are generated in a probabilistic framework ideally with an appreciation of both semicontinuous and fully continuous probabilistic approaches. KEY FEATURES:• The first book to focus entirely on DNA mixtures and the complexities involved with interpreting the results • Takes a hands-on approach offering theory with worked examples and exercises to be easily understood and implementable by laboratory personnel • New methods heretofore unpublished previously provide a means to innovate deconvoluting a mixed DNA profile assign an LR and appropriately report the weight of evidence • Includes a chapter on assigning LRs for close relatives (i. e. “It’s not me it was my brother”) and discusses strategies for the validation of probabilistic genotyping softwareForensic DNA Profiling fills the void for labs unfamiliar with LRs and moving to probabilistic solutions and for labs already familiar with LRs but wishing to understand how they are calculated in more detail. The book will be a welcome read for lab professionals and technicians students and legal professionals seeking to understand and apply the techniques covered. | Forensic DNA Profiling A Practical Guide to Assigning Likelihood Ratios

GBP 44.99
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Test Quality for Construction Materials and Structures Proceedings of the International RILEM/ILAC Symposium

Skin Diseases of the Dog and Cat

Skin Diseases of the Dog and Cat

A Colour Handbook of Skin Diseases of the Dog and Cat was one of the first books to bring key information about skin diseases to clinicians in an easy-to-use problem-oriented format. This fully revised and updated Third Edition responds to the huge growth in knowledge about skin conditions over the last decade including the discovery of new conditions the development of new approaches to management and effective new treatment options. Chapters are organized based on symptoms each containing a decision tree giving basic and practical guidance. The clear user-friendly design provides one condition per page (or spread of pages). 13 chapters covering over 120 skin claw and ear conditions classified by their principle presenting sign. Concise systematically structured text covering definition aetiology and pathogenesis clinical features differential diagnoses diagnostic tests and management. Flow charts in each chapter to help clinicians get to the right diagnosis. Special focus on diseases affecting paediatric patients as well as chapters discussing paw ear and nasal planum diseases. Explanation of new treatments for atopic dermatitis. Over 350 superb colour photographs and diagrams mostly new for this this edition. A focus on clinical practice and the need to explain the disease to the owner. Up-to-date and fully referenced throughout. This practical book continues to provide an entirely comprehensive guide to the diagnosis and management of veterinary skin conditions in a format that is easily accessible for busy clinicians.

GBP 51.99
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Handbook of Mitochondrial Dysfunction

Genomes 5

Genomes 5

Genomes 5 has been completely revised and updated. It is a thoroughly modern textbook about genomes and how they are investigated. As with previous Genomes editions techniques come first then genome anatomies followed by genome function and finally genome evolution. The genomes of all types of organism are covered: viruses bacteria fungi plants and animals including humans and other hominids. Genome sequencing and assembly methods have been thoroughly revised to include new developments in long-read DNA sequencing. Coverage of genome annotation emphasizes genome-wide RNA mapping with CRISPR-Cas 9 and GWAS methods of determining gene function covered. The knowledge gained from these techniques forms the basis of the chapters that describe the three main types of genomes: eukaryotic prokaryotic (including eukaryotic organelles) and viral (including mobile genetic elements). Coverage of genome expression and replication is truly genomic concentrating on the genome-wide implications of DNA packaging epigenome modifications DNA-binding proteins non-coding RNAs regulatory genome sequences and protein-protein interactions. Also included are examples of the applications of metabolomics and systems biology. The final chapter is on genome evolution including the evolution of the epigenome using genomics to study human evolution and using population genomics to advance plant breeding. Established methods of molecular biology are included if they are still relevant today and there is always an explanation as to why the method is still important. Genomes 5 is the ideal text for upper-level courses focused on genomes and genomics. Key Features A highly accessible and well-structured book with chapters organized into four parts to aid navigation Superb artwork illustrates the key concepts and mechanisms Each chapter has a set of short-answer questions and in-depth problems to test the reader’s understanding of the material Thoroughly up to date with references to the latest research from the 2020s

GBP 64.99
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Manipulation of the Avian Genome

Forensic Biology

A Hands-On Introduction to Forensic Science Cracking the Case Second Edition

A Hands-On Introduction to Forensic Science Cracking the Case Second Edition

A Hands-On Introduction to Forensic Science Second Edition continues in the tradition of the first edition taking a wholly unique approach to teaching forensic science. Each chapter begins with a brief fictional narrative that runs through the entire book; it is a crime fiction narrative that describes the interaction of a veteran homicide detective teamed with a criminalist and the journey they take together to solve a missing persons case. Step-by-step the book progressive reveals pieces of information about the crime followed by the more traditional presentation of scientific principles and concepts on a given forensic topics. Each chapter concludes with a series of user friendly cost effective hands-on lab activities that provide the students the skills necessary to analyze the evidence presented in each chapters. The new edition is completely updated with special focus on new DNA techniques in DNA sequencing DNA phenotyping and bioinformatics. Students will engage in solving a missing persons case by documenting the crime scene analyzing physical evidence in the lab and presenting findings in a mock trial setting. Within the chapters themselves students learn about the technical forensic concepts presented within each of the opening stories segments. The book culminates with having the students playing to role of the main characters in a trial—attorneys scientific experts suspect judge bailiff and jury—to present and judge the evidence in a mock trial setting. The mock trial will mimic what takes place in a real courtroom and the jury of swill be asked to deliberate on the evidence presented to determine the guilt or innocence of the suspect. | A Hands-On Introduction to Forensic Science Cracking the Case Second Edition

GBP 59.99
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The Complete Guide to the ABC Molecular Biology Certification Exam

The Complete Guide to the ABC Molecular Biology Certification Exam

In 2009 the National Academy of Sciences (NAS) authored the report Strengthening Forensic Science in the United States: A Path Forward. In it the Committee expressed the need for accreditation and certification. Accreditation long recognized by public labs as an important benchmark in quality was recognized as an important way to standardize laboratories that provide forensic services. Certification can play an important role as a method of oversight in the forensic sciences—something also recommended by the - National Commission on Forensic Science in October 2014. The Complete Guide to the ABC's Molecular Biology is a professional certification examination preparation text for forensic scientists taking the American Board of Criminalistics Examination in Molecular Biology. The book serves as a resource for forensic scientists—who are facing more and more pressure to become certified—to support them in their pursuit of forensic certification. In the years since the NAS report was published there has been increased discussion of forensic certification requirements. ABC’s Molecular Biology exam is a quality certification and learning the concepts for it will invariably help any professional working in the field. The book prepares readers in all relevant topic areas including: accreditation safety biological screen principles anatomy and cell biology crime scene and evidence handling concepts in genetics biochemistry statistics DNA evidence and DNA testing. The book will be particularly helpful for forensic science laboratory technicians police and investigations professionals forensic serology and DNA analysts attorneys and forensic science students. This study guide follows the guidelines for the exam and presents all the information necessary to prepare individuals to pass the exam. | The Complete Guide to the ABC Molecular Biology Certification Exam

GBP 74.99
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Prenatal Medicine

Nutrients and Epigenetics

Phylogenomics A Primer

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced and processes seamlessly integrated. This microscale advantage translates into greater sensitivity more accurate results and better information. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology its limitations and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods microfluidic operations chemical separations sample preparation and injection methods detection technology and various chemical and biological analyses. Other topics of interest include the use of polymeric chips fluid flow valve and control single-cell analysis DNA and RNA amplification techniques DNA hybridization immunoassays and enzymatic assays. Originally conceived as a single chapter published in Ewing's Analytical Instrumentation this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.

GBP 44.99
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Next-Generation Sequencing Data Analysis

Next-Generation Sequencing Data Analysis

Next-generation DNA and RNA sequencing has revolutionized biology and medicine. With sequencing costs continuously dropping and our ability to generate large datasets rising data analysis becomes more important than ever. Next-Generation Sequencing Data Analysis walks readers through next-generation sequencing (NGS) data analysis step by step for a wide range of NGS applications. For each NGS application this book covers topics from experimental design sample processing sequencing strategy formulation to sequencing read quality control data preprocessing read mapping or assembly and more advanced stages that are specific to each application. Major applications include: RNA-seq: Both bulk and single cell (separate chapters) Genotyping and variant discovery through whole genome/exome sequencing Clinical sequencing and detection of actionable variants De novo genome assembly ChIP-seq to map protein-DNA interactions Epigenomics through DNA methylation sequencing Metagenome sequencing for microbiome analysis Before detailing the analytic steps for each of these applications the book presents introductory cellular and molecular biology as a refresher mostly for data scientists the ins and outs of widely used NGS platforms and an overview of computing needs for NGS data management and analysis. The book concludes with a chapter on the changing landscape of NGS technologies and data analytics. The second edition of this book builds on the well-received first edition by providing updates to each chapter. Two brand new chapters have been added to meet rising data analysis demands on single-cell RNA-seq and clinical sequencing. The increasing use of long-read sequencing has also been reflected in all NGS applications. This book discusses concepts and principles that underlie each analytic step along with software tools for implementation. It highlights key features of the tools while omitting tedious details to provide an easy-to-follow guide for practitioners in life sciences bioinformatics biostatistics and data science. Tools introduced in this book are open source and freely available.

GBP 74.99
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Manual of Forensic Science An International Survey

Handbook Of Applied Econometrics And Statistical Inference

Fluorescence Sensors and Biosensors

Fluorescence Sensors and Biosensors

Fluorescence-based sensing is a significant technique used in prominent fields such as fluorescence-activated cell sorting DNA sequencing high-throughput screening and clinical diagnostics. Fluorescence Sensors and Biosensors emphasizes the most recent developments and emerging technologies with the broadest impacts. The text begins with the development of aptamers (oligoribonucleotides) and biorecognition techniques based on periplasmic binding proteins. The following chapters review the molecular beacon approach for DNA recognition describe resonance energy transfer (FRET) in sensing and present the use of carbonic anhydrase recognition platform for metal ion determination and imaging. The book explores the advantages of fluorophores fluorescent labels sensor and assay construction metal-enhanced fluorescence phosphorescent labels and lab-on-a-chip applications. It also describes new anion-selective fluorescent probes used as analytes in clinical determinations. The final chapters highlight the application of fluorescence sensing technology to several practical problems such as the development of planar waveguide biosensors for clinical diagnostics and the adaptation of fluorescence-based sensing approaches for biochemical production by fermentation. The book also discusses the measurement of analytes such as free zinc ions at ultratrace levels in biological specimens. Written by internationally renowned authors in their fields Fluorescence Sensors and Biosensors provides an up-to-date account of fluorescence-based sensors focused on practical applications in biotechnology analytical chemistry and biomedicine.

GBP 59.99
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Reliability Analysis with Minitab

Reliability Analysis with Minitab

Statistical Analysis for the Reliability Engineering ProfessionalEffectively conduct reliability analysis using the world’s leading statistical software. Reliability Analysis with Minitab® outlines statistical concepts and applications explains the theory of probability reliability analysis and quality improvement and provides step-by-step instruction on the use of Minitab. Minitab introduces reliability analysis tools that can be used to perform tasks that range from checking the distribution fit of lifetime data to estimating the warranty costs of a product. Perform the Analyses Needed to Minimize Product Failures and Reduce CostsChock full of examples that include numerous case studies and over 200 screenshots this book is a comprehensive guide to quality and reliability in the service and manufacturing industries. It illustrates the shapes of the most commonly used statistical distributions in reliability analysis and in simple language demonstrates concepts that include parametric reliability analysis nonparametric reliability analysis warranty analysis accelerated life testing reliability test plans and probit analysis. Illustrating the application of Minitab for reliability analysis this book explains how to: Perform reliability analysis of a product with right-censored and exact failure time data Complete reliability analysis of a product with arbitrarily censored failure time dataAchieve nonparametric reliability analysis of a productPredict the amount of money that is needed to cover the warranty costs for products in a specific period of time in the futureAnalyze the results from accelerated life testing on two different productsDetermine the reliability test sample size when the test time and the number of failures are constrainedRegulate the testing time when test sample size and the number of failures are constrainedCompare the reliabilities of parts from different vendorsTest whether the reliability of a product depends on certain factorsPredict the stress level at which a product will fail after a certain test period

GBP 44.99
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