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Protein Purification

Protein Purification

This second edition of Protein Purification provides a guide to the major chromatographic techniques including non-affinity absorption techniques affinity procedures non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic starting with the properties of water progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment followed by discussions and examples of each technique and their applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates and postgraduates who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification. Key Features * Guide to the major techniques used in protein purification * Includes flowcharts to help the reader slect the best purification strategy * Contains step-by-step protocols that guide the reader through each technique and its use * Includes exercises and solutions

GBP 66.99
1

The Fluorescent Protein Revolution

The Fluorescent Protein Revolution

Advances in fluorescent proteins live-cell imaging and superresolution instrumentation have ushered in a new era of investigations in cell biology medicine and physiology. From the identification of the green fluorescent protein in the jellyfish Aequorea victoria to the engineering of novel fluorescent proteins The Fluorescent Protein Revolution explores the history properties and applications of these important probes. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers several cutting-edge imaging applications. These include superresolution microscopy of cellular fine structures FRET microscopy to visualize protein interactions and cell-signaling activities inside living cells photobleaching and photoactivation techniques to visualize protein behaviors techniques that exploit plant and algal photoreceptors to enable light-regulated control of enzymatic activities and the noninvasive imaging of tumor–host interactions in living animals. In color throughout this book presents the fundamental principles and latest advances in the field including the associated development of imaging techniques that exploit fluorescent proteins. It is accessible to a broad audience from optical imaging experts to novices needing an introduction to the field.

GBP 59.99
1

Peptide and Protein Drug Analysis

Peptide and Protein Drug Analysis

Furthering efforts to simulate the potency and specificity exhibited by peptides and proteins in healthy cells this remarkable reference supplies pharmaceutical scientists with a wealth of techniques for tapping the enormous therapeutic potential of these molecules-providing a solid basis of knowledge for new drug design. Provides a broad comprehensive overview of peptides and proteins as mediators of cell movement proliferation differentiation and communication. Written by more than 50 leading international authorities Peptides and Protein Drug Analysisdiscusses strategies for dealing with the complexity of peptides and proteins in conformational flexibility and amino acid sequence variability analyzes drug formulations facilitated by solid-phase peptide synthesis and recombinant DNA technology examines chemical purity analysis by high-pressure chromatographic capillary electrophoretic gel electrophoretic and isoelectric focusing methods highlights drug design elements derived from protein folding bioinformatics and computational chemistry demonstrates uses of unnatural mutagenesis and combinatorial chemistry explores mass spectrometry protein sequence and carbohydrate analysis illustrates bioassays and other new functional analysis methods surveys spectroscopic techniques such as ultraviolet fluorescence Fourier transform infrared and nuclear magnetic resonance (NMR) addresses ways of distinguishing between levels of therapeutic and endogenous agents in cells reviews structural analysis tools such as ultracentrifugation and light X-ray and neutron scattering and more! Featuring over 3400 bibliographic citations and more than 500 tables equations and illustrations Peptide and Protein Drug Analysis is a must-read resource for pharmacists; pharmacologists; analytical organic and pharmaceutical chemis

GBP 59.99
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Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

The Latest Developments on the Role of Dynamics in Protein Functions Computational Approaches to Protein Dynamics: From Quantum to Coarse-Grained Methods presents modern biomolecular computational techniques that address protein flexibility/dynamics at all levels of theory. An international contingent of leading researchers in chemistry physics and biology show how these advanced methods provide insights into dynamic aspects of biochemical processes. A particular focus is on intrinsically disordered proteins (IDPs) which lack a well-defined three-dimensional structure and function as dynamic ensembles. The book covers a wide spectrum of dynamics from electronic structure-based to coarse-grained techniques via multiscaling at different levels. After an introduction to dynamics and historical overview of basic methodologies the book addresses the following issues: Is there a quantitative relationship between enzymatic catalysis and protein dynamics? Which are the functionally relevant motions of proteins? How can structural properties and partner recognition mechanisms of IDPs be simulated? How can we speed up molecular dynamics? How can we describe conformational ensembles by the synergistic effort of computations and experiments? While dynamics is now considered essential for interpreting protein action it is not yet an integral component in establishing structure–function relationships of proteins. Helping to reshape this classical view in biochemistry this groundbreaking book explores advances in computational methodology and contributes to the new ensemble way of studying proteins. | Computational Approaches to Protein Dynamics From Quantum to Coarse-Grained Methods

GBP 69.99
1

Phage Display In Biotechnology and Drug Discovery

Phage Display In Biotechnology and Drug Discovery

Phage Display in Biotechnology and Drug Discovery Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition the book discusses current theories principles and methods in the field and demonstrates applications for peptide phage display protein phage display and the development of novel antibodies. The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery. Key Features: Describes the basic principles of phage display and the methods and systems that have been developed for key applications Outlines applications for peptide phage display in the development of vaccines and high- throughput screens as well as the exploration of protein–protein interactions and enzyme substrate characteristics Details the use of phage display in the engineering of protein stability the identification and mapping of protein–protein interactions and the development of catalysts Provides broad coverage of the impact of phage display technology on the development of protein therapeutics Presents expert opinions on future challenges in the field

GBP 69.99
1

A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery

A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery

The development of suitable assays the integration of appropriate technology and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery integrates the experience of diverse experts who offer fundamental and practical guidance across numerous situations. The book first discusses assay developments for important target classes such as protein kinases and phosphatases proteases nuclear receptors G protein-coupled receptors ion channels and heat shock proteins. It next examines assay developments for cell viability apoptosis and infectious diseases. The contributors explore the application of emerging technologies and systems including image-based high content screening RNA interference and primary cells. Finally they discuss the essential components of the integrated HTS process such as screening automation compound library management the screening of natural products from botanical sources and screening informatics. Designed to motivate researchers to bring further advances to the field this volume provides practical guidance on how to initiate validate optimize and manage a bioassay intended to screen large collections of compounds. Drawing on the knowledge from experts actively involved in assay development and HTS this is a resource that is both comprehensive and focused.

GBP 59.99
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Knowledge Discovery in Proteomics

Knowledge Discovery in Proteomics

Multi-modal representations the lack of complete and consistent domain theories rapid evolution of domain knowledge high dimensionality and large amounts of missing information - these are challenges inherent in modern proteomics. As our understanding of protein structure and function becomes ever more complicated we have reached a point where the actual management of data is a major stumbling block to the interpretation of results from proteomic platforms to knowledge discovery. Knowledge Discovery in Proteomics presents timely authoritative discussions on some of the key issues in high-throughput proteomics exploring examples that represent some of the major challenges of knowledge discovery in the field. The authors focus on five specific domains:Mass spectrometry-based protein analysisProtein-protein interaction network analysisSystematic high-throughput protein crystallizationSystematic integrated analysis of multiple data repositoriesSystems biologyIn each area the authors describe the challenges created by the type of data produced and present potential solutions to the problem of data mining within the domain. They take a systems approach covering individual data and integrating its computational aspects from data preprocessing storage and access to analysis visualization and interpretation. With clear exposition practical examples and rich illustrations this book presents an outstanding overview of this emerging field and builds the background needed for the fruitful exchange of ideas between computational and biological scientists.

GBP 59.99
1

Introduction to Computational Proteomics

Introduction to Computational Proteomics

Introduction to Computational Proteomics introduces the field of computational biology through a focused approach that tackles the different steps and problems involved with protein analysis classification and meta-organization. The book starts with the analysis of individual entities and works its way through the analysis of more complex entities from protein families to interactions cellular pathways and gene networks. The first part of the book presents methods for identifying the building blocks of the protein space such as motifs and domains. It also describes algorithms for assessing similarity between proteins based on sequence and structure analysis as well as mathematical models such as hidden Markov models and support vector machines that are used to represent protein families and classify new instances. The second part covers methods that investigate higher order structure in the protein space through the application of unsupervised learning algorithms such as clustering and embedding. The book also explores the broader context of proteins. It discusses methods for analyzing gene expression data predicting protein-protein interactions elucidating cellular pathways and reconstructing gene networks. This book provides a coherent and thorough introduction to proteome analysis. It offers rigorous formal descriptions along with detailed algorithmic solutions and models. Each chapter includes problem sets from courses taught by the author at Cornell University and the Technion. Software downloads data sets and other material are available at biozon. org

GBP 59.99
1

Nanotechnology in Drug Delivery Fundamentals Design and Applications

Nanotechnology in Drug Delivery Fundamentals Design and Applications

This important new book provides the fundamental understanding of the peptide and protein drug delivery systems with a special focus on their nanotechnology applications. Addressing an increasing interest in peptide and protein drug delivery systems in both academic and industrial circles worldwide this book fills the need for a comprehensive review and assessment of conventional and nonconventional routes of administration. | Nanotechnology in Drug Delivery Fundamentals Design and Applications

GBP 82.99
1

High Voltage Engineering

High Voltage Engineering

Inspired by a new revival of worldwide interest in extra-high-voltage (EHV) and ultra-high-voltage (UHV) transmission High Voltage Engineering merges the latest research with the extensive experience of the best in the field to deliver a comprehensive treatment of electrical insulation systems for the next generation of utility engineers and electric power professionals. The book offers extensive coverage of the physical basis of high-voltage engineering from insulation stress and strength to lightning attachment and protection and beyond. Presenting information critical to the design selection testing maintenance and operation of a myriad of high-voltage power equipment this must-have text: Discusses power system overvoltages electric field calculation and statistical analysis of ionization and breakdown phenomena essential for proper planning and interpretation of high-voltage tests Considers the breakdown of gases (SF6) liquids (insulating oil) solids and composite materials as well as the breakdown characteristics of long air gaps Describes insulation systems currently used in high-voltage engineering including air insulation and insulators in overhead power transmission lines gas-insulated substation (GIS) and cables oil-paper insulation in power transformers paper-oil insulation in high-voltage cables and polymer insulation in cables Examines contemporary practices in insulation coordination in association with the International Electrotechnical Commission (IEC) definition and the latest standards Explores high-voltage testing and measuring techniques from generation of test voltages to digital measuring methods With an emphasis on handling practical situations encountered in the operation of high-voltage power equipment High Voltage Engineering provides readers with a detailed real-world understanding of electrical insulation systems including the various factors affecting—and the actual means of evaluating—insulation performance and their application in the establishment of technical specifications.

GBP 74.99
1

High Power Microwaves

High Power Microwaves

Following in the footsteps of its popular predecessors High Power Microwaves Third Edition continues to provide a wide-angle integrated view of the field of high power microwaves (HPMs). This third edition includes significant updates in every chapter as well as a new chapter on beamless systems that covers nonlinear transmission lines. Written by an experimentalist a theorist and an applied theorist respectively the book offers complementary perspectives on different source types. The authors address:How HPM relates historically and technically to the conventional microwave fieldThe possible applications for HPM and the key criteria that HPM devices have to meet in order to be appliedHow high power sources work including their performance capabilities and limitationsThe broad fundamental issues to be addressed in the future for a wide variety of source typesThe book is accessible to several audiences. Researchers currently in the field can widen their understanding of HPM. Present or potential users of microwaves will discover the advantages of the dramatically higher power levels that are being made available. Newcomers to the field can pursue further research. Decision makers in direct energy acquisition and related fields such as radar communications and high energy physics can see how developments in HPM will affect them.

GBP 44.99
1

Introduction to Proteins Structure Function and Motion Second Edition

Introduction to Proteins Structure Function and Motion Second Edition

Introduction to Proteins provides a comprehensive and state-of-the-art introduction to the structure function and motion of proteins for students faculty and researchers at all levels. The book covers proteins and enzymes across a wide range of contexts and applications including medical disorders drugs toxins chemical warfare and animal behavior. Each chapter includes a Summary Exercises and References. New features in the thoroughly-updated second edition include: A brand-new chapter on enzymatic catalysis describing enzyme biochemistry classification kinetics thermodynamics mechanisms and applications in medicine and other industries. These are accompanied by multiple animations of biochemical reactions and mechanisms accessible via embedded QR codes (which can be viewed by smartphones) An in-depth discussion of G-protein-coupled receptors (GPCRs) A wider-scale description of biochemical and biophysical methods for studying proteins including fully accessible internet-based resources such as databases and algorithms Animations of protein dynamics and conformational changes accessible via embedded QR codes Additional features Extensive discussion of the energetics of protein folding stability and interactions A comprehensive view of membrane proteins with emphasis on structure-function relationship Coverage of intrinsically unstructured proteins providing a complete realistic view of the proteome and its underlying functions Exploration of industrial applications of protein engineering and rational drug design Each chapter includes a Summary Exercies and References Approximately 300 color images Downloadable solutions manual available at www. crcpress. com For more information including all presentations tables animations and exercises as well as a complete teaching course on proteins' structure and function please visit the author's website. Praise for the first edition This book captures in a very accessible way a growing body of literature on the structure function and motion of proteins. This is a superb publication that would be very useful to undergraduates graduate students postdoctoral researchers and instructors involved in structural biology or biophysics courses or in research on protein structure-function relationships. David Sheehan ChemBioChem 2011 Introduction to Proteins is an excellent state-of-the-art choice for students faculty or researchers needing a monograph on protein structure. This is an immensely informative thoroughly researched up-to-date text with broad coverage and remarkable depth. Introduction to Proteins would provide an excellent basis for an upper-level or graduate course on protein structure and a valuable addition to the libraries of professionals interested in this centrally important field. Eric Martz Biochemistry and Molecular Biology Education 2012 | Introduction to Proteins Structure Function and Motion Second Edition

GBP 84.99
1

High Temperature Electronics

High Temperature Electronics

The development of electronics that can operate at high temperatures has been identified as a critical technology for the next century. Increasingly engineers will be called upon to design avionics automotive and geophysical electronic systems requiring components and packaging reliable to 200 °C and beyond. Until now however they have had no single resource on high temperature electronics to assist them. Such a resource is critically needed since the design and manufacture of electronic components have now made it possible to design electronic systems that will operate reliably above the traditional temperature limit of 125 °C. However successful system development efforts hinge on a firm understanding of the fundamentals of semiconductor physics and device processing materials selection package design and thermal management together with a knowledge of the intended application environments. High Temperature Electronics brings together this essential information and presents it for the first time in a unified way. Packaging and device engineers and technologists will find this book required reading for its coverage of the techniques and tradeoffs involved in materials selection design and thermal management and for its presentation of best design practices using actual fielded systems as examples. In addition professors and students will find this book suitable for graduate-level courses because of its detailed level of explanation and its coverage of fundamental scientific concepts. Experts from the field of high temperature electronics have contributed to nine chapters covering topics ranging from semiconductor device selection to testing and final assembly.

GBP 59.99
1

Plant Biotechnology Volume 1 Principles Techniques and Applications

Plant Biotechnology Volume 1 Principles Techniques and Applications

This book first of this new two-volume set provides an informative tour of the basics of biotechnology to recent advances in biotechnology. Knowledge of new and fresh approaches is a prerequisite to solving plant biological problems and to this end the editors have brought together a group of contributors who address the most recent techniques and their applications in plant biotechnology. The chapters discuss some recent techniques such as TILLING (Targeting Induced Local Lesions In Genomes) advances in molecular techniques to study diversity protein purification and methods and analysis in protein-protein interaction detection. The volume also covers molecular markers and QTL mapping including four chapters that deal with different molecular markers development of mapping populations and association mapping for dissecting the genetic basis of complex traits in plants in sufficient detail. The knowledge of biotechnology techniques and their applications will be valuable for researchers and scientists as well as for the many students engaged in plant biotechnology studies. | Plant Biotechnology Volume 1 Principles Techniques and Applications

GBP 82.99
1

Tubular String Characterization in High Temperature High Pressure Oil and Gas Wells

Tubular String Characterization in High Temperature High Pressure Oil and Gas Wells

High temperature high oil pressure oil and gas well completion testing have always been a technical challenge and basic theoretical research is one of the key factors needed to ensure a successful completion test. The completion test basic theory includes: a stress analysis of the completion string completion string buckling behavior and temperature and pressure distribution prediction. The completion string is the main bearing and power transmission component for oil and gas well operations and production and it is required to take on a combination of loads which result in completion string deformation. Because of these complex relationships completion string stress analysis has become increasingly more complicated. This book discusses the characters of tubular strings in HTHP (High Temperature - High Pressure) oil and gas wells. These characters include the mechanical behavior of tubular strings and the temperature and pressure variation of tubular strings in different conditions. Mathematical models are established for different conditions and solution existence and uniqueness of some models is discussed providing algorithms corresponding to the different models. Numerical experiments are presented to verify the validity of models and the feasibility of algorithms and the impact of the parameters of models for oil and gas wells is also discussed. This book is written for production and testing engineers to provide them with the tools to deal more effectively with the numerical decisions they have to take and for researchers and technicians in petroleum and gas testing and production engineering. Finally it is also intended to serve as a reference book for mathematicians college teachers and students. | Tubular String Characterization in High Temperature High Pressure Oil and Gas Wells

GBP 84.99
1

High-Intensity Ultrasonics Theory and Industrial Applications

High-Intensity Ultrasonics Theory and Industrial Applications

Presenting a comprehensive description of the theory and physics of high-intensity ultrasound this book also deals with a wide range of problems associated with the industrial applications of ultrasound mainly in the areas of metallurgy and mineral processing. The book is divided into three sections and Part I introduces the reader to the theory and physics of high-intensity ultrasound. Part II considers the design of ultrasonic generators mechanoacoustic radiators and other vibrational systems as well as the control of acoustic parameters when vibrations are passed into a processed medium. Finally Part III describes problems associated with various uses of high-intensity ultrasound in metallurgy. The applications of high-intensity ultrasound for metal shaping thermal and thermochemical treatment welding cutting refining and surface hardening are also discussed here. This comprehensive monograph will provide an invaluable source of information which has been largely unavailable in the West until now. | High-Intensity Ultrasonics Theory and Industrial Applications

GBP 56.99
1

Nutritional Guidelines for Athletic Performance The Training Table

Nutritional Guidelines for Athletic Performance The Training Table

Knowing the basic nutrition requirements and combining them with proper training are the two most important factors for athletes to achieve peak performance levels. Nutritional Guidelines for Athletic Performance: The Training Table addresses these needs on a comprehensive basis from a training table perspective. Offering practical guidelines for practitioners and athletes the book focuses on the key macronutrients that fuel daily metabolism and exercise training and explores differing needs for various athletes and their individual goals. Topics discussed include:General principles and physiology of caloric intake for all major macronutrients and how this pertains to both active and sedentary individualsEnergy demands and nutritional requirements for strength-power athletes and elite competitors in endurance sports Protein carbohydrate fat vitamin and mineral needs of athletesThe importance of optimal fluid and hydration during exerciseHow to determine body weight for a desired body fat percentage recommended calorie consumption dietary suggestions and useful meal planning tools for a wide variety of caloric needsThe difference between energy intake and energy expenditure and how athletes can eat to build muscle lose fat and optimize performanceScientific strategies on how to time exercise and food intake to more effectively replenish glycogen increase protein synthesis and blunt protein degradationHow the physiological changes that accompany aging change nutrient needs and guidelines for older athletes on maximizing performance and maintaining healthStructuring a diet containing appropriate amounts of macronutrients (protein carbohydrates and fat) and micronutrients (vitamins and minerals) can be a daunting task. This text adequately addresses how athletes can do so in an effe | Nutritional Guidelines for Athletic Performance The Training Table

GBP 59.99
1

Group Theory for High Energy Physicists

Group Theory for High Energy Physicists

Although group theory has played a significant role in the development of various disciplines of physics there are few recent books that start from the beginning and then build on to consider applications of group theory from the point of view of high energy physicists. Group Theory for High Energy Physicists fills that role. It presents groups especially Lie groups and their characteristics in a way that is easily comprehensible to physicists. The book first introduces the concept of a group and the characteristics that are imperative for developing group theory as applied to high energy physics. It then describes group representations since matrix representations of a group are often more convenient to deal with than the abstract group itself. With a focus on continuous groups the text analyzes the root structure of important groups and obtains the weights of various representations of these groups. It also explains how symmetry principles associated with group theoretical techniques can be used to interpret experimental results and make predictions. This concise gentle introduction is accessible to undergraduate and graduate students in physics and mathematics as well as researchers in high energy physics. It shows how to apply group theory to solve high energy physics problems.

GBP 59.99
1

Treatment programmes for high risk offenders

Treatment programmes for high risk offenders

High risk offenders can have a disproportionate impact on their communities because despite all manner of sentencing options they continue to commit a wide range of crimes both minor and serious. It is tempting to throw the book at them sometimes even to throw away the key. However anything that helps offenders to change their propensity for re-offending can really make a difference. Over the last 30 years scientific research has guided the provision of treatment rehabilitation and reintegration services that lead to reductions in re-offending. Much of what we know however comes from work with medium-risk offenders. Although this work is important and valuable there is a lower level of complexity to working with medium-risk offenders than most high-risk offenders require. This book recognizes the need to research and develop different approaches to rehabilitating high-risk offenders. Each of the contributions takes a different approach with a different group of offenders in a different setting. Cumulatively the chapters provide encouragement for those working with high risk offenders along with a wide range of ideas about how to develop better services. This book was originally published as a special issue of Psychology Crime & Law. | Treatment programmes for high risk offenders

GBP 46.99
1

Introduction to High-Dimensional Statistics

Introduction to High-Dimensional Statistics

Praise for the first edition: [This book] succeeds singularly at providing a structured introduction to this active field of research. … it is arguably the most accessible overview yet published of the mathematical ideas and principles that one needs to master to enter the field of high-dimensional statistics. … recommended to anyone interested in the main results of current research in high-dimensional statistics as well as anyone interested in acquiring the core mathematical skills to enter this area of research. —Journal of the American Statistical Association Introduction to High-Dimensional Statistics Second Edition preserves the philosophy of the first edition: to be a concise guide for students and researchers discovering the area and interested in the mathematics involved. The main concepts and ideas are presented in simple settings avoiding thereby unessential technicalities. High-dimensional statistics is a fast-evolving field and much progress has been made on a large variety of topics providing new insights and methods. Offering a succinct presentation of the mathematical foundations of high-dimensional statistics this new edition: Offers revised chapters from the previous edition with the inclusion of many additional materials on some important topics including compress sensing estimation with convex constraints the slope estimator simultaneously low-rank and row-sparse linear regression or aggregation of a continuous set of estimators. Introduces three new chapters on iterative algorithms clustering and minimax lower bounds. Provides enhanced appendices minimax lower-bounds mainly with the addition of the Davis-Kahan perturbation bound and of two simple versions of the Hanson-Wright concentration inequality. Covers cutting-edge statistical methods including model selection sparsity and the Lasso iterative hard thresholding aggregation support vector machines and learning theory. Provides detailed exercises at the end of every chapter with collaborative solutions on a wiki site. Illustrates concepts with simple but clear practical examples.

GBP 74.99
1

Multi-purpose High-rise Towers and Tall Buildings

Multi-purpose High-rise Towers and Tall Buildings

Interest continues to develop in the design and construction of high-rise towers and tall buildings structures with heights ranging from 75m to 500m and even more. This volume presents the papers from the third in a series of international conferences on the subject organised by the International Federation of High-rise Structures. The papers have been drawn together under the grand theme of the Conquest of Vertical Space in the 21st Century. The conference has been organised by the UK's Concrete Society and sponsored by the IFHS and the Council on Tall Buildings and Urban Habitat and the Fdration Internationale de la Prcontrainte (FIP). This prestigious collaboration has brought forth a body of high quality practical and research papers. | Multi-purpose High-rise Towers and Tall Buildings

GBP 59.99
1